E-GEOD-48827 - Structural basis of a novel Interferon b signaling axis mediated via the ifnar1 receptor

Status
Released on 13 July 2013, last updated on 13 May 2014
Organism
Mus musculus
Samples (12)
Array (1)
Protocols (7)
Description
Type I interferons (IFNs) are a family of cytokines that play an important role in regulating immune responses to pathogens and tumors and are used therapeutically. All IFNs are considered to signal via the heterodimeric IFNAR1-IFNAR2 complex, yet some subtypes such as IFN? can exhibit distinct functional properties, although the molecular basis of this is unclear. Here we demonstrate IFN uniquely and specifically ligates to IFNAR1 in an IFNAR2-independent manner and provide the structural basis of the IFNAR1-IFN interaction. We show that the IFNAR1-IFN complex transduces signals to modulate the expression of a set of genes independently of IFNAR2. Moreover, we show the in vivo importance of the IFNAR1-IFN signaling axis in a murine model of LPS-induced septic shock. Thus, we provide a molecular basis for understanding specific functions of IFN?. Interferon b induced gene expression in peritoneal exudate cells was measured 3hr post intra-peritoneal injection of 10,000IU/ml of interferon beta or saline into wildtype and Ifnar2-/- mice. Three independant experiments were performed for each treatment in both genotypes using different mice for each sample.
Experiment type
transcription profiling by array 
Contacts
Sam Forster <geo@ncbi.nlm.nih.gov>, Jodee Gould, Nicole De Weerd, Paul Hertzog
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-48827.idf.txt
Sample and data relationshipE-GEOD-48827.sdrf.txt
Raw data (1)E-GEOD-48827.raw.1.zip
Processed data (1)E-GEOD-48827.processed.1.zip
Array designA-MEXP-724.adf.txt
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