E-GEOD-48660 - An improved SELEX-Seq strategy for characterizing DNA-binding specificity of transcription factor: NF-κB as an example

Status
Released on 5 May 2014, last updated on 2 June 2014
Organism
Homo sapiens
Samples (5)
Protocols (2)
Description
We provided an improved SELEX-Seq strategy for characterizing DNA-binding specificity of transcription factor. We valided the strategy by characterzing the DNA-binding specificty of NF-κB p50 dimer. Proteins of the Nf-kappab family were bound to DNA oligonucleotides containing a degenerate region. The protein-DNA complexes were selected after one or multiple rounds of SELEX and the DNA molecules were deep sequenced.
Experiment type
ChIP-seq 
Contacts
Jinke Wang <wangjinke@seu.edu.cn>, Guangming Gu, Hinxin Xu, Tingting Wang, Yang Yang
Citation
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
Files
Investigation descriptionE-GEOD-48660.idf.txt
Sample and data relationshipE-GEOD-48660.sdrf.txt
Processed data (1)E-GEOD-48660.processed.1.zip
Links