E-GEOD-48421 - Genome-wide analyses of the DRB response of the histone H3 Lys36 methylation state, as well as Ash1l occupancy in mouse embryonic stem cells

Released on 20 November 2013, last updated on 27 November 2013
Mus musculus
Samples (10)
Protocols (4)
Using wild type and Ash1l ∆SET mutant embryonic stem cells, against a prevalent notion, here we report di-,tri-methylation of histone H3 Lys36 occur independently of ongoing transcription. Ash1l ∆SET mutant shows the impaired methylation levels in broad range of genome. Intriguingly, data implicate that a binding of retinoic acid receptor to a certain genomic region promotes installation of the tri-methylation in the retinoic acid receptor-associated gene independent of its ongoing transcription. Examination of 2 different histone modifications in 2 cell types in 2 different conditions. Examination of Ash1l occupancy in 2 different conditions.
Experiment type
Takaho A. Endo <takaho.endo@riken.jp>, Jafar Sharif, Ken Higashimoto, Kenichi Nishioka, Takaho A Endo
Ash1l methylates lys36 of histone h3 independently of transcriptional elongation to counteract polycomb silencing. Miyazaki H, Higashimoto K, Yada Y, Endo TA, Sharif J, Komori T, Matsuda M, Koseki Y, Nakayama M, Soejima H, Handa H, Koseki H, Hirose S, Nishioka K. , Europe PMC 24244179
Exp. designProtocolsVariablesProcessedSeq. reads
Investigation descriptionE-GEOD-48421.idf.txt
Sample and data relationshipE-GEOD-48421.sdrf.txt
Processed data (1)E-GEOD-48421.processed.1.zip