E-GEOD-48197 - Different DNA methylation between hypothalamus paraventricular (PVH) and ventromedial (VMH) nuclei

Status
Released on 1 October 2013, last updated on 3 May 2014
Organism
Mus musculus
Samples (4)
Array (1)
Protocols (5)
Description
The goal is to compare the DNA methylation patterns between ARH and PVH to examine to what extent DNA methylation is region specific in genome scale. ARH and PVH were micro-dissected from mice brains. Each 7 pieces of ARH or PVH were pooled and used for DNA methylation comparison by methylation specific amplification microarray (MSAM). As a brief description of the MSAM: 500ng of genomic DNA was serially digested with SmaI and XmaI followed by an adaptor ligation and adaptor mediated PCR amplification and then cohybridization. Two-color cohybridizations were performed as paired comparison of PVH vs. ARH with two biological replicates including dye swap.
Experiment type
transcription profiling by array 
Contacts
Ge Li <geo@ncbi.nlm.nih.gov>, Natalia Matalan-Hung, Richard B Simerly, Robert A Waterland, Wenjuan Zhang
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-48197.idf.txt
Sample and data relationshipE-GEOD-48197.sdrf.txt
Raw data (1)E-GEOD-48197.raw.1.zip
Processed data (1)E-GEOD-48197.processed.1.zip
Array designA-GEOD-17331.adf.txt
Links