6 protocols
AccessionNameType
P-GSE48079-6
array scanning protocol
Standard Illumina scanning protocol
P-GSE48079-1
normalization data transformation protocol
Illumina Genome Studio software (v.1.9.0) was used for data processing: background subtraction and Quantile normalization ID_REF = VALUE = quantile normalized Detection Pval =
P-GSE48079-5
hybridization protocol
Biotinylated cRNA were hybridized to the Illumina array per manufacturer's instructions
P-GSE48079-4
labelling protocol
Biotinylated cRNA were prepared according to the standard Illumina protocol
P-GSE48079-2
growth protocol
Monolayer cells were maintained in DMEM supplemented with 10% heat-inactivated fetal bovine serum), 100 units/mL penicillin, and 100 μg/mL streptomycin. Spheroid cells were maintained in ultra-low adhesion flasks using serum-free media supplemented with B27, L-Glutamine, Pen/Strep, hEGF (20 ng/ml), and hFGFb (40ng/ml).
P-GSE48079-3
nucleic acid extraction protocol
Total RNA was prepared using RNeasy mini kit (Qiagen) according to the manufacturer's instructions