7 protocols
AccessionNameType
P-GSE48057-1
normalization data transformation protocol
Intensities of arrays have been quantile-normalized using Partek Genomic Suite (v6.6). Differential expressions were calculated using ANOVA of Partek package. ID_REF = VALUE = Log2 GC-RMA signal intensity
P-GSE48057-7
array scanning protocol
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
P-GSE48057-6
hybridization protocol
Following labeling, cDNAs were hybridized for 16 hr at 45C on Mouse Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
P-GSE48057-5
labelling protocol
Biotinylated cDNAs were prepared according to the standard Ambion and Affymetrix protocol from 250 ng total RNA (The Ambion WT Expression Kit and GeneChip Terminal Labeling Kit, Affymetrix).
P-GSE48057-4
nucleic acid extraction protocol
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
P-GSE48057-2
sample treatment protocol
The experiment was set up in a 6 well plates with 3 wells as control untreated cells, and 3 well SSRI treated cells. Seeding density was 50,000 cells per well.
P-GSE48057-3
growth protocol
MC3T3-E1 cells purchased from ATCC (Manassas, VA) were reconstituted and grown in a T-75 flask until transfer into T-175 flask. Cells were cultured in alpha minimum Eagles medium (αMEM) supplemented with penicillin/streptomycin (pen/strep) and 1% fetal bovine serum (FBS).