6 protocols
AccessionNameType
P-GSE48054-1
normalization data transformation protocol
Data was extracted using Agilent Feature Extraction software 9.5. and statistical analysis was conducted using AgiMicroRna package in R (Bioconductor package) ID_REF = VALUE = normalized signal intensity
P-GSE48054-6
array scanning protocol
Scanning of the slides was done using Genepix 4000B (Molecular devices)
P-GSE48054-5
hybridization protocol
Hybridization was done at 55 degrees for 18 hours.
P-GSE48054-4
labelling protocol
Labeling of RNA was done following Agilent hydridization kit following manufacturer's guidelines
P-GSE48054-3
nucleic acid extraction protocol
Total RNA was isolated using STAT60 method. Total RNA was assessed for quality using Bioanalyzer.
P-GSE48054-2
sample treatment protocol
Zebrafish embryos were exposed to vehicle control (ethanol) or valproic acid starting from 4 hours post-fertilization (hpf) to 96 hpf. Embryos were sampled at 48 and 96 hpf for miRNA expression profiling