E-GEOD-4799 - Transcription profiling of spermatogonial stem cells established from 6 day old male mice to identify GDNF regulated genes by comparing cells in the presence, absence and timed replacement of GDNF

Status
Submitted on 9 May 2006, released on 24 November 2007, last updated on 27 March 2012
Organism
Mus musculus
Samples (15)
Array (1)
Protocols (5)
Description
GDNF-regulated gene expression was studied in cultures of actively self-renewing spermatogonial stem cells established from 6 day old male mice. GDNF is the essential growth factor regulating mouse spermatogonial stem cell self-renewal. Using a serum-free chemically defined culture system that supports mouse spermatogonial stem cell self-renewal for extended periods of time, GDNF-regulated genes were identified using microarray profiling. Experiment Overall Design: Established cultures of highly enriched self-renewing spermatogonial stem cells were subjeted to withrawal of GDNF and GFRalpha1 for 18-hr followed by replacement of the growth factors for 2, 4, and 8-hr. Gene expression was studied using microarray profiling prior to withdrawal, after withdrawal and at each time-point of GDNF/GFRalpha1 replacement.
Experiment types
transcription profiling by array, co-expression, compound treatment, growth condition, time series
Contact
Citation
Identifying genes important for spermatogonial stem cell self-renewal and survival. Jon M Oatley, Mary R Avarbock, Aino I Telaranta, Douglas T Fearon, Ralph L Brinster. Proc Natl Acad Sci U S A 103(25):9524-9 (2006)
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-4799.idf.txt
Sample and data relationshipE-GEOD-4799.sdrf.txt
Processed data (1)E-GEOD-4799.processed.1.zip
Array designA-AFFY-45.adf.txt
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