E-GEOD-47873 - Gene expression profiles MCF-10A cells overexpressing MBD2
Released on 17 June 2013, last updated on 3 June 2014
Methylated DNA binding protein 2 (MBD2) has been shown to bind specific methylated promoters and suppress transcription. Here we systematically investigate MBD2 suppression by overexpressing MBD2 in MCF-10A cells and generating gene expression profiles of overexpressing cells and normal MCF-10A cells. MCF-10A cells were infected with MBD2 lentivirus in order to increase MBD2 expression. Total RNA was extracted from both infected and non-infected cells and hybridized to Affymetrix gene expression microarrays. Three technical replicates were hybridized for infected and non-infected cells.
transcription profiling by array
Johanne Wyglinski, Matthew Suderman, Moshe Szyf, Sebastian Alvarado, Stephen Andrews