E-GEOD-47873 - Gene expression profiles MCF-10A cells overexpressing MBD2

Status
Released on 17 June 2013, last updated on 3 June 2014
Organism
Homo sapiens
Samples (6)
Array (1)
Protocols (7)
Description
Methylated DNA binding protein 2 (MBD2) has been shown to bind specific methylated promoters and suppress transcription. Here we systematically investigate MBD2 suppression by overexpressing MBD2 in MCF-10A cells and generating gene expression profiles of overexpressing cells and normal MCF-10A cells. MCF-10A cells were infected with MBD2 lentivirus in order to increase MBD2 expression. Total RNA was extracted from both infected and non-infected cells and hybridized to Affymetrix gene expression microarrays. Three technical replicates were hybridized for infected and non-infected cells.
Experiment type
transcription profiling by array 
Contacts
Johanne Wyglinski, Matthew Suderman, Moshe Szyf, Sebastian Alvarado, Stephen Andrews
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-47873.idf.txt
Sample and data relationshipE-GEOD-47873.sdrf.txt
Raw data (1)E-GEOD-47873.raw.1.zip
Processed data (1)E-GEOD-47873.processed.1.zip
Array designA-AFFY-44.adf.txt
Links