E-GEOD-4785 - Transcription profiling of Jurkat CF4+ T cells transfected with simian immunodeficiency virus Nef cells leads to a molecular profile of viral persistence and immune evasion
Submitted on 5 May 2006, released on 26 October 2007, last updated on 27 March 2012
Jurkat T cell line was transfected with SIV Nef controlled by an inducible promoter system. Nef was induced by addition of 10 micromolar pronasterone A and gene expression values were determined after 24 hrs. Control Jurkat cells were untransfected and treated with 10 micromolar pronasterone A and gene expressio values were determined after 24 hrs. Experiment Overall Design: Experimental conditions: 24 hr SIV Nef expression at physiological concentrations in T cells. Experiment Overall Design: Controls: Non-transfected. 24 hr.
transcription profiling by array, co-expression, compound treatment, in vitro
Expression of simian immunodeficiency virus Nef protein in CD4+ T cells leads to a molecular profile of viral persistence and immune evasion. Thomas Ndolo, Michael George, Hau Nguyen, Satya Dandekar. Virology 353(2):374-87 (2006)