E-GEOD-47643 - Gene expression of in vitro cultivated preB cells before and after 8, 16 and 24 hours induction of miR-221

Status
Released on 5 June 2013, last updated on 3 June 2014
Organism
Mus musculus
Samples (12)
Array (1)
Protocols (7)
Description
PreB cells were analyzed for differences in gene expression before and after the overexpression of miR-221. In order to dissect possible targets for the miR-221, gene expression profiles of preB cells un-induced or induced for the miR-221 expression after 8, 16 and 24 hours were compared. All induction time-points, e.g. after 8, 16 and 24 hours were compared to un-induced preB cells and to each other group. Gene expression profiles of un-induced preB cells, preB cells induced for miR-221 expression after 8, 16, and 24 hours were analyzed using Affymetrix MG 430 2.0 whole genome arrays. Each time-point was performed in triplicates for un-induced preB cells and preB cells induced for miR-221 expression after 8, 16, and 24 hours (12 arrays in total). To obtain genes significantly downregulated upon induction of miR-221, the expression profiles of un-induced preB cells were compared to preB cells activated for 8, 16, or 24 h and were also compared to each other. After total RNA extraction, reverse transcription, cDNA extraction, the biotinylated cRNA was transcribed, fragmented, and 15 µg cRNA hybridized in triplicates for each of the four groups to the 12 GeneChip arrays: Group1, un-induced preB cells, Group2, preB cells induced for miR-221 expression after 8h, Group3, preB cells induced for miR-221 expression after 16h, Group4, preB cells induced for miR-221 expression after 24h.
Experiment type
transcription profiling by array 
Contacts
Joachim R. Grün <Gruen@DRFZ.de>, Corinne Bouquet, Fritz Melchers, Joachim R Grün, Marko Knoll, Szandor Simmons
Citations
SiPaGene: A new repository for instant online retrieval, sharing and meta-analyses of GeneChip expression data. Menssen A, Edinger G, Gr�n JR, Haase U, Baumgrass R, Gr�tzkau A, Radbruch A, Burmester GR, H�upl T. , Europe PMC 19265543
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-47643.idf.txt
Sample and data relationshipE-GEOD-47643.sdrf.txt
Raw data (1)E-GEOD-47643.raw.1.zip
Processed data (1)E-GEOD-47643.processed.1.zip
Array designA-AFFY-45.adf.txt
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