E-GEOD-4764 - Transcription profiling of mouse Peyers patch and mesenteric lymph nodes after oral infection with Y. enterolitica

Status
Submitted on 2 May 2006, released on 24 November 2007, last updated on 3 May 2014
Organism
Mus musculus
Samples (15)
Array (1)
Protocols (1)
Description
To gain a comprehensive view of the host response to pathogens within these tissues, we determined the transcriptional profiles of intestinal lymphatic tissue infected with Y. enterocolitica. Expression analysis using Affymetrix GeneChips revealed a complex host response in the Peyer’s patches (PP) and mesenteric lymph nodes (MLN) following oral infection with Y. enterocolitica. Peyer's patches and mesenteric lymph nodes were surgically removed from uninfected (control) and infected (experimental) mice at 12 hours, day 2, 4 and 7 post oral infection. Tissue samples from 10 mice per time-point were combined. Total RNA was extracted using the Trizol method prior to total cDNA syntesis, labeling and hybridization to Affymetrix MGU74Av2 GeneChips. Data was processed and analyzed using MAS 5.0 and custom analysis protocols. Samples from Peyer's patches and mesenteric lymph nodes are included in this series.
Experiment types
transcription profiling by array, co-expression, disease state, in vivo, organism part comparison, time series
Contact
Citation
Histamine signaling through the H(2) receptor in the Peyer's patch is important for controlling Yersinia enterocolitica infection. Scott A Handley, Peter H Dube, Virginia L Miller. Proc Natl Acad Sci U S A 103(24):9268-73 (2006)
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-4764.idf.txt
Sample and data relationshipE-GEOD-4764.sdrf.txt
Processed data (1)E-GEOD-4764.processed.1.zip
Array designA-AFFY-6.adf.txt
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