5 protocols
AccessionType
normalization data transformation protocol
Data was normalized using Robust Multichip Averaging (RMA) algorithm and baseline transformation to the median of all samples using GeneSpring 6.0 software. ID_REF = VALUE = RMA Normalized signal intensity
array scanning protocol
GeneChips were scanned using Affymetrix® Scanner 3000 7G.
hybridization protocol
In accordance with manufacturer's protocols
labelling protocol
In accordance with manufacturer's protocols
nucleic acid extraction protocol
Extraction of total RNA using total RNA purification kit (Norgen Biotek, Canada) was performed according to the manufacturer's instructions.