E-GEOD-47354 - Genome-wide expression profiling of Hic-5 and TGFB in WPMY fibroblasts

Status
Released on 26 August 2013, last updated on 2 September 2013
Organism
Homo sapiens
Samples (20)
Array (1)
Protocols (7)
Description
Identifying the effect of the co-regulator Hic-5 (TGFB1I1) and TGFB on the transcriptional profile of WPMY human prostate fibroblast cells with view to further elucidating the broader biological role of Hic-5 and TGFB on fibroblast. Knockdown of Hic5 for 48 hours in PShTert-AR cells significantly altered the androgen regulation of approximately 1347 genes. The effect of Hic-5 knockdown was to suppress androgen regulation of approximately 85% of those transcripts, by either reducing androgen mediated repression or activition of gene expression. Isogenic WPMY-1 fibroblasts stably transfected with short-hairpin RNA against Hic-5 or transfected with non-specific negative control short-hairpin RNA, were kept in 5% hormone stripped FBS RPMI for 48 hours and subsequently treated with either ethanol vehicle control or TGFB for 16hr. Total RNA was extracted. Five independent vehicle and five TGFB siSCR and siHic5 samples were hybrydised to Affymetrix Human Gene 1.0 ST array chips.
Experiment type
transcription profiling by array 
Contacts
damien leach <damien.leach@adelaide.edu.au>, Damien A Leach, Grant Buchanan
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-47354.idf.txt
Sample and data relationshipE-GEOD-47354.sdrf.txt
Raw data (1)E-GEOD-47354.raw.1.zip
Processed data (1)E-GEOD-47354.processed.1.zip
Array designA-AFFY-141.adf.txt
Links