E-GEOD-47215 - The Obesity-associated Fto gene regulates activity of the dopaminergic midbrain circuitry [WT]

Status
Released on 15 June 2013, last updated on 4 July 2013
Organism
Mus musculus
Samples (6)
Protocols (4)
Description
Here we use MeRIP-Seq to analyze global adenosine methylation (m6A) in mRNAs in the midbrain and striatum of Fto-deficient mice. We find that Fto deficiency leads to increased methylation within a subset of mRNAs important for neuronal signaling, including many within the dopaminergic signaling pathway. Collectively, our results show that Fto regulates demethylation of specific mRNAs in vivo, and this activity relates to control of dopaminergic transmission. Profiling of m6A in midbrain and striatum from wild type mice
Experiment types
other, methylation profiling by high throughput sequencing 
Contacts
Christopher Mason <geo@ncbi.nlm.nih.gov>, Bengt F Belgardt, Hella S Brönneke, Jens C Brüning, Kate D Meyer, Linda A Verhagen, Linda Koch, Marcelo O Dietrich, Martin E Hess, Olivier Elemento, Peter Kloppenburg, Sabine D Jordan, Samie R Jaffrey, Simon Hess, Tamas L Horvath, Thomas Franz, Ulrich Rüther
MINSEQE
Exp. designProtocolsFactorsProcessedSeq. reads
Files
Investigation descriptionE-GEOD-47215.idf.txt
Sample and data relationshipE-GEOD-47215.sdrf.txt
Links