E-GEOD-47045 - Development pathway for skin resident memory CD103+CD8+ T cells

Status
Released on 1 October 2013, last updated on 7 October 2013
Organism
Mus musculus
Samples (24)
Array (1)
Protocols (5)
Description
Tissue resident memory T cells (TRM) provide superior protection against infection localised to extra-lymphoid compartments in the body. Here we show that CD103+CD8+ TRM cells develop in skin from killer cell lectin-like receptor (KLR)G1-negative precursors that selectively infiltrate the epithelial layer. In the skin, a combination of chemokine-guided epithelial entry, local interleukin (IL)-15 and transforming growth factor (TGF)-β signalling is required for formation and survival of these long-lived memory cells. Importantly, TRM differentiation results in the gradual acquisition of a unique transcriptional profile that differs from that expressed by memory cells in the circulation and other types of skin-resident intra-epithelial T cells, such as the dendritic epidermal T cells (DETC). We provide a comprehensive molecular and developmental framework for the local differentiation of a distinct type of peripheral memory T cell that contributes to an important first-line of immune defence in barrier tissues such as skin and mucosa. 24 samples were analyzed: 3 replicates of memory gB-T CD8+. CD103+ T cells isolated from the skin of C57/BL6 mice on day 30 p.i. with HSV KOS. 3 replicates of memory P14 CD8+ T cells isolated from gut of mice on day 60 p.i. with LCMV Armstrong. 3 replicates of memory gB-T CD8+ T cells from the lung of mice on day 30 p.i. with influenza WSN. 3 replicates of memory CD62L high CD8+ T cells from the spleen of mice on day 30 p.i. with HSV KOS. 3 replicates of memory CD62L low CD8+ T cells from the spleen of mice of day 30 p.i. with HSV KOS. 3 replicates of γδ-DETC isolated from the skin of C57/BL6 mice on day 30 p.i. with HSV KOS. 3 replicates of αβ-DETC from naive TCRδ-/- mice; and 3 replicates of naive gB-T CD8+ T cells from the spleen of naive gB-T transgenic mice.
Experiment type
transcription profiling by array 
Contacts
Joel Zhi-Iong Ma <jzma@unimelb.edu.au>, A Rahimpour, A T Stock, D C Tscharke, F R Carbone, J Vega-Ramos, J Z Ma, L K Mackay, M Inouye, M L Hafon, N Collins, P Lauzurica, T Gebhardt, T Stefanovic, W R Heath
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-47045.idf.txt
Sample and data relationshipE-GEOD-47045.sdrf.txt
Raw data (1)E-GEOD-47045.raw.1.zip
Processed data (1)E-GEOD-47045.processed.1.zip
Array designA-AFFY-130.adf.txt
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