E-GEOD-46967 - Comprehensive gene expression profile of extrahepatic bile ducts in mice with experimental biliary atresia
Released on 1 June 2013, last updated on 17 June 2013
Newborn Balb/c mice were injected intraperitoneally with 1.5x10^6 fluorescent-forming units (ffu) of type- A Rhesus Rotavirus (RRV) or 0.9% normal saline (NS; control) within 24 hours of birth to induce experimental model of biliary atresia. Extrahepatic bile ducts including gallbladder were microdissected en bloc at 3, 7 and 14 days after RRV or saline injections. GeneChip® Mouse Gene 1.0 ST Array (Affymetrix, CA) were used to screen mRNAs whose expression was differently regulated after RRV challenge compared to normal saline controls. Gene expression profiling: Each experimental conditon contains 3 sets of samples. Each sample consists of two to six extrahepatic bile ducts pooled prior to isolation of total RNA to ensure generation of adequate mRNA to perform quantitative experiments.
transcription profiling by array
Anil G Jegga, John McGee, Jorge A Bezerra, Kazuhiko Bessho, Marepalli Rao, Pranavkumar Shivakumar, Reena Mourya, Stephanie Walters