E-GEOD-4696 - Transcription profiling by array of mouse primary dermal fibroblasts after treatment with 5-aza-2'-deoxycytidine

Status
Released on 23 November 2007, last updated on 3 June 2014
Organism
Mus musculus
Samples (6)
Array (1)
Protocols (7)
Description
DNA methylation can contribute to the stable transcriptional silencing of mammalian genes. Oftentimes, these genes are important developmental regulators, and their silencing in cell types where they are not supposed to be active is important for the phenotypic stability of the cells. To identify key developmental regulator genes whose expression in terminally differentiated cells may be inhibited by DNA methylation, mouse dermal fibroblasts were demethylated with 5-aza-2’-deoxycytidine, and changes in gene expression monitored by microarray analysis. Three biological replicates for both control and 5-aza-2'-deoxycytidine treatment were derived. Cells were primary mouse dermal fibroblasts derived by explant procedure separately for each biological replicate. Treated cells were exposed to 5uM 5-aza-2'-deoxycytidine for 96 hours with recovery in normal medium for 24 hours. Control cells were untreated.
Experiment types
transcription profiling by array, co-expression, compound treatment
Contact
Citation
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-4696.idf.txt
Sample and data relationshipE-GEOD-4696.sdrf.txt
Processed data (1)E-GEOD-4696.processed.1.zip
Array designA-AFFY-45.adf.txt
Links