E-GEOD-46921 - SLE serum induced transcripts regulated by IFNa2b

Status
Released on 3 June 2014, last updated on 7 June 2014
Organism
Homo sapiens
Samples (4)
Arrays (2)
Protocols (7)
Description
To explore the full extent of IFN-regulated transcriptional changes, we exposed monocytes from two healthy donors to recombinant type I IFN (IFN-α2b) in vitro. RNA was extracted at 6 hrs and the expression data was normalized to that of monocytes cultured with medium. Blood monocytes isolated from healthy volunteers were incubated with 20% autologous serum alone or in the presence of 1000 U/ml of IFNα2b (Schering Plough, Kenilworth, NJ) in 6-well plates at a concentration of 106 monocytes per well in 3 ml of media. After incubation for six hours at 37 ⁰C, cells were harvested and RNA was extracted.
Experiment type
transcription profiling by array 
Contacts
Nicole Baldwin <geo@ncbi.nlm.nih.gov>, Alicia Rodriquez-Pla, Alisa Gotte, Holden Maecker, Jacques Banchereau, Jose Rossello-Urgell, Karolina A Palucka, Lorien Nassi, Lynda Bennett, Marilynn Punaro, Pinakeen Patel, Victoria Cantrell, Virginia Pascual
Citation
IFN Priming Is Necessary but Not Sufficient To Turn on a Migratory Dendritic Cell Program in Lupus Monocytes. Rodriguez-Pla A, Patel P, Maecker HT, Rossello-Urgell J, Baldwin N, Bennett L, Cantrell V, Baisch J, Punaro M, Gotte A, Nassi L, Wright T, Palucka AK, Banchereau J, Pascual V. , Europe PMC 24829414
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-46921.idf.txt
Sample and data relationshipE-GEOD-46921.sdrf.txt
Raw data (1)E-GEOD-46921.raw.1.zip
Processed data (1)E-GEOD-46921.processed.1.zip
Array designsA-AFFY-33.adf.txt, A-AFFY-34.adf.txt
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