E-GEOD-46917 - Differentially expressed transcripts in SLE blood monocytes according to their capacity to induce mixed lymphocytic reaction

Status
Released on 3 June 2014, last updated on 7 June 2014
Organism
Homo sapiens
Samples (46)
Arrays (2)
Protocols (7)
Description
We screened SLE monocytes from 19 SLE patients and selected 4 that induced CD4+ T cell proliferation in vitro and 4 that did not. CFSE labeled CD4-T cells (105) were incubated with SLE monocytes (2 x 104). Cells were harvested at 6 hours for RNA extraction. We screened SLE monocytes from 19 SLE patients and selected 4 that induced CD4+ T cell proliferation in vitro and 4 that did not. CFSE labeled CD4-T cells (105) were incubated with SLE monocytes (2 x 104). Cells were harvested at 6 hours for RNA extraction.
Experiment type
transcription profiling by array 
Contacts
Nicole Baldwin <geo@ncbi.nlm.nih.gov>, Alicia Rodriquez-Pla, Alisa Gotte, Holden Maecker, Jacques Banchereau, Jose Rossello-Urgell, Karolina A Palucka, Lorien Nassi, Lynda Bennett, Marilynn Punaro, Pinakeen Patel, Victoria Cantrell, Virginia Pascual
Citation
IFN Priming Is Necessary but Not Sufficient To Turn on a Migratory Dendritic Cell Program in Lupus Monocytes. Rodriguez-Pla A, Patel P, Maecker HT, Rossello-Urgell J, Baldwin N, Bennett L, Cantrell V, Baisch J, Punaro M, Gotte A, Nassi L, Wright T, Palucka AK, Banchereau J, Pascual V. , Europe PMC 24829414
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-46917.idf.txt
Sample and data relationshipE-GEOD-46917.sdrf.txt
Raw data (1)E-GEOD-46917.raw.1.zip
Processed data (1)E-GEOD-46917.processed.1.zip
Array designsA-AFFY-33.adf.txt, A-AFFY-34.adf.txt
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