E-GEOD-4675 - Transcription profiling by array of mouse pre-frontal cortex at different time-points after birth

Status
Released on 15 May 2006, last updated on 1 May 2015
Organism
Mus musculus
Samples (23)
Array (1)
Protocols (3)
Description
Abnormal development of the prefrontal cortex (PFC) is associated with a number of neuropsychiatric disorders that have an onset in childhood or adolescence. Although the basic laminar structure of the PFC is established in utero, extensive remodeling continues into adolescence. To map the overall pattern of changes in cortical gene transcripts during post-natal development, we made serial measurements of mRNA levels in mouse PFC using oligonucleotide microarrays. We observed changes in mRNA transcripts consistent with known post-natal morphological and biochemical events. Overall, most transcripts that changed significantly showed a progressive decrease in abundance after birth, with the majority of change between post-natal weeks 2 and 4. Genes with cell proliferative, cytoskeletal, extracellular matrix, plasma membrane lipid / transport, protein folding, and regulatory functions had decreases in mRNA levels. Quantitative PCR verified the microarray results for six selected genes: DNA methyltransferase 3A (Dnmt3a), procollagen, type III, alpha 1 (Col3a1), solute carrier family 16 (monocarboxylic acid transporters), member 1 (Slc16a1), MARCKS-like 1 (Marcksl1), nidogen 1 (Nid1) and 3-hydroxybutyrate dehydrogenase (heart, mitochondrial) (Bdh). Keywords: time course, development, mRNA expression Single-channel affymetrix arrays were used to profile mRNA expression in the prefrontal cortex (PFC) of male mice at different time-points after birth (post-natal). Each array is an independent animal.
Experiment type
transcription profiling by array 
Contacts
Paul C Boutros <Paul.Boutros@utoronto.ca>, Albert H Wong, Allan B Okey, Hubert H Van Tol, Mawahib O Semeralul, Olga Likhodi
Citation
Microarray analysis of the developing cortex. Semeralul MO, Boutros PC, Likhodi O, Okey AB, Van Tol HH, Wong AH.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-4675.idf.txt
Sample and data relationshipE-GEOD-4675.sdrf.txt
Raw data (1)E-GEOD-4675.raw.1.zip
Processed data (2)E-GEOD-4675.processed.1.zip, E-GEOD-4675.processed.2.zip
Array designA-AFFY-23.adf.txt
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