E-GEOD-46684 - Expression data: Venous malformation-causative TIE2-mutations mediate an AKT-dependent decrease in PDGFB

Status
Released on 4 June 2013, last updated on 3 June 2014
Organism
Homo sapiens
Samples (12)
Array (1)
Protocols (7)
Description
Comparison of transcriptional profiles of human umbilical vein endothelial cells (HUVECs) expressing wild-type vs. VM-causative mutant forms of TIE2/TEK. The effects of the most common Venous Malformation-causative mutations in the endothelial cell tyrosine kinase receptor: R849W and L914F, were tested. 743 genes were differentially expressed across the four groups. The 80 genes distinguishing between L914F and wild-type TIE2-expressing HUVECs were analyzed in greater detail. 3 batches each of: non-transfected, and wild-type TIE2, R849W-TIE2, and L914F-TIE2 overexpressing HUVECs were compared by exon-array profiling.
Experiment type
transcription profiling by array 
Contacts
Melanie Uebelhoer, Miikka Vikkula, Nisha Limaye
Citation
Venous malformation-causative TIE2 mutations mediate an AKT-dependent decrease in PDGFB. Uebelhoer M, N�tynki M, Kangas J, Mendola A, Nguyen HL, Soblet J, Godfraind C, Boon LM, Eklund L, Limaye N, Vikkula M. , Europe PMC 23633549
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-46684.idf.txt
Sample and data relationshipE-GEOD-46684.sdrf.txt
Raw data (2)E-GEOD-46684.raw.1.zip, E-GEOD-46684.raw.2.zip
Processed data (1)E-GEOD-46684.processed.1.zip
Array designA-GEOD-17120.adf.txt
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