E-GEOD-46661 - Over-expression of bHLH122 in Arabidopsis

Status
Released on 7 May 2013, last updated on 3 June 2014
Organism
Arabidopsis thaliana
Samples (4)
Array (1)
Protocols (7)
Description
bHLH122 could be induced by salt, osmotic and drought stress except ABA, the transgentic Arabidopsis were more tolerant to abiotic stresses, such as drought and salt. What's more, in the overexpression plants, the endogenesis ABA contents were higher than WT. We found there existed an interaction between bHLH122 and CYP707A3 by virtue of EMSA and ChIP assays. We wanted to learn more about the molecular mechanism of bHLH122 and to explore what had changed in the over-expression plant through Genechips. Two independent overexpression bHLH122 lines and WT Arabidopsis were chosen to RNA extraction and hybridization on Affymetrix microarrays. The plants were sowed on MS medium after 96 h stratification,then transferred into soil after one weeks. After 2 weeks, collected the shoot above the soil and extracted RNA, digested by DNaseⅠand hybrid on Affymetrix microarrays.
Experiment type
transcription profiling by array 
Contacts
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-46661.idf.txt
Sample and data relationshipE-GEOD-46661.sdrf.txt
Raw data (1)E-GEOD-46661.raw.1.zip
Processed data (1)E-GEOD-46661.processed.1.zip
Array designA-AFFY-2.adf.txt
Links