E-GEOD-46542 - Focal amplification of HOXD-harboring chromosome region is implicated in multiple-walled carbon nanotubes-induced carcinogenicity

Status
Released on 2 October 2013, last updated on 3 June 2014
Organism
Homo sapiens
Samples (14)
Array (1)
Protocols (7)
Description
Amplificaition of HOXD9 and HOXD13 genes was found in MWCNTs induced carcinogencity. By overexpression or silence of of HOXD9 and HOXD13 gene may alter tumorigenicity. To investigate the realted signaling pathways invovled in HOXD regulated tumorigencity, we analyzed the gene expression profile of the HOXD9 or HOXD13 overexpression HEK293 cells, which compared to vehicle expressing cells HEK293 cells was transfected with HA-tag-HOXD9 and V5-tag HOXD13 vector, and further selected by G418. Three independnent clones and mix clones of each genes were selected, and compared to corresponding tag control cell. (HA-HOXD9-mix, a, b, c v.s HA-MOCK-1, 2, 3) (V5-HOXD13-mix, a, b, c v.s V5-MOCK-1, 2, 3)
Experiment type
transcription profiling by array 
Contacts
Ping Wu <m102094020@tmu.edu.tw>, Chao-Chi Ho, Huei-Wen Chen, Ker-Chau Li, Pan-Chyr Yang, Shin-Sheng Yuan, Wan-Yu Hsieh, Wei Chen
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-46542.idf.txt
Sample and data relationshipE-GEOD-46542.sdrf.txt
Raw data (1)E-GEOD-46542.raw.1.zip
Processed data (1)E-GEOD-46542.processed.1.zip
Array designA-AFFY-44.adf.txt
Links