E-GEOD-46515 - Expression data from mouse model using targeted deletion of hepatic RICTOR (Albumin-Cre Rictor LoxP/LoxP)

Status
Released on 23 September 2013, last updated on 13 July 2015
Organism
Mus musculus
Samples (8)
Array (1)
Protocols (5)
Description
Recent work using mouse models has revealed that mTORC2, which unlike mTORC1 is not acutely sensitive to rapamycin, plays a key role in the regulation of organismal physiology. The substrates and pathways regulated by mTORC2 are at present relatively unknown Using a mouse model with a targeted deletion of hepatic RICTOR, we investigated the loss of mTORC2 on the murine liver transcriptome Rictor floxed (RKO) and control mice (n=4 per group) were fasted overnight, refed for 3 hr, then sacrificed. Livers were removed, rinsed in PBS, and flash frozen in liquid nitrogen. RNA was extracted and hybridized to Affymetrix Genechip Mouse Gene 1.0 ST arrays
Experiment type
transcription profiling by array 
Contacts
Joan M Boylan <geo@ncbi.nlm.nih.gov>, David M Sabatini, Dudley W Lamming, Philip A Gruppuso
Citation
Hepatic signaling by the mechanistic target of rapamycin complex 2 (mTORC2). Lamming DW, Demirkan G, Boylan JM, Mihaylova MM, Peng T, Ferreira J, Neretti N, Salomon A, Sabatini DM, Gruppuso PA.
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-46515.idf.txt
Sample and data relationshipE-GEOD-46515.sdrf.txt
Raw data (1)E-GEOD-46515.raw.1.zip
Processed data (1)E-GEOD-46515.processed.1.zip
Array designA-AFFY-130.adf.txt
Links