E-GEOD-46340 - RNA-seq reveals changes in sphingolipid metabolism upon transformation
Released on 17 October 2013, last updated on 25 November 2013
We sequenced mRNA from three independent pairs of mock and c-SRC transformed mouse embryonic fibroblasts and examined changes in expression of sphingolipid metabolism genes Three independent cultures of NIH3T3 MEFs were retrovirally transduced with either pBabe-Puro, og pBabe-Puro containing c-srcY527F. After a 7 day of selection in puromycine the three vector and three src transduced pools were propagated for RNA sequencing.
RNA-seq of coding RNA
Mikkel Rohde <firstname.lastname@example.org>, Marja Jäättelä
Transformation-associated changes in sphingolipid metabolism sensitize cells to lysosomal cell death induced by inhibitors of acid sphingomyelinase. Petersen NH, Olsen OD, Groth-Pedersen L, Ellegaard AM, Bilgin M, Redmer S, Ostenfeld MS, Ulanet D, Dovmark TH, Lï¿½nborg A, Vindelï¿½v SD, Hanahan D, Arenz C, Ejsing CS, Kirkegaard T, Rohde M, Nylandsted J, Jï¿½ï¿½ttelï¿½ M. , Europe PMC 24029234