E-GEOD-46340 - RNA-seq reveals changes in sphingolipid metabolism upon transformation

Status
Released on 17 October 2013, last updated on 25 November 2013
Organism
Mus musculus
Samples (6)
Protocols (3)
Description
We sequenced mRNA from three independent pairs of mock and c-SRC transformed mouse embryonic fibroblasts and examined changes in expression of sphingolipid metabolism genes Three independent cultures of NIH3T3 MEFs were retrovirally transduced with either pBabe-Puro, og pBabe-Puro containing c-srcY527F. After a 7 day of selection in puromycine the three vector and three src transduced pools were propagated for RNA sequencing.
Experiment type
RNA-seq of coding RNA 
Contacts
Mikkel Rohde <mr@cancer.dk>, Marja Jäättelä
Citation
Transformation-associated changes in sphingolipid metabolism sensitize cells to lysosomal cell death induced by inhibitors of acid sphingomyelinase. Petersen NH, Olsen OD, Groth-Pedersen L, Ellegaard AM, Bilgin M, Redmer S, Ostenfeld MS, Ulanet D, Dovmark TH, L�nborg A, Vindel�v SD, Hanahan D, Arenz C, Ejsing CS, Kirkegaard T, Rohde M, Nylandsted J, J��ttel� M. , Europe PMC 24029234
MINSEQE
Exp. designProtocolsFactorsProcessedSeq. reads
Files
Investigation descriptionE-GEOD-46340.idf.txt
Sample and data relationshipE-GEOD-46340.sdrf.txt
Links