E-GEOD-46317 - Drosophila CNS glial microarray

Status
Released on 24 April 2013, last updated on 3 June 2014
Organism
Drosophila melanogaster
Samples (9)
Array (1)
Protocols (7)
Description
Gliogenesis in the Drosophila CNS occurs during embryogenesis and also during the postembryonic larval stages. Several glial subtypes are generated in the postembryonic CNS through the proliferation of differentiated glial cells. The genes and molecular pathways that regulate glial proliferation in the postembryonic CNS are poorly understood. In this study we aimed to use gene expressing profiling of CNS tissue enriched in glia to identify genes expressed in glial cells in the postembryonic CNS. We used microarrays to compare the gene expression profiles from the larval CNS of animals that had increased numbers of glial cells to identify genes that are expressed in glia. RNA was purified from the late third instar larval CNS from control larvae, or larvae expressing an activated form of the FGF receptor (Hlt[ACT]), or overexpressing the insulin receptor (InR) in glial cells using the glial specific driver repoGal4 to increase the number of glial cells and generate CNS tissue enriched in glia.
Experiment type
transcription profiling by array 
Contacts
Joseph M. Bateman <joseph_matthew.bateman@kcl.ac.uk>, Amelie Avet-Rochex, Joseph M Bateman
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-46317.idf.txt
Sample and data relationshipE-GEOD-46317.sdrf.txt
Raw data (1)E-GEOD-46317.raw.1.zip
Processed data (1)E-GEOD-46317.processed.1.zip
Array designA-AFFY-35.adf.txt
Links