E-GEOD-46315 - Engineering Bone Tissue Substitutes from Human Induced Pluripotent Stem Cells

Status
Released on 24 April 2013, last updated on 3 June 2014
Organism
Homo sapiens
Samples (12)
Array (1)
Protocols (5)
Description
Global gene expression data of human embryonic stem cell-, human induced pluripotent stem cell- and bone marrow-derived mesenchymal progenitor cells before and after culture onto osteoinductive scaffolds in perfusion bioreactors. The hypothesis tested in the present study was that perfusion culture in bioreactors influenced the expression levels of several genes involved in proliferation and osteogenic differentiation. Results provide important information of the response of human embryonic stem cell-, human induced pluripotent stem cell- and bone marrow-derived mesenchymal progenitor cell to osteogenic stimulation under perfusion cultures, such as genes involved in cell proliferation and division as well as osteogenic differentiation and bone development. Total RNA obtained from human embryonic stem cell-, human induced pluripotent stem cell- and bone marrow-derived mesenchymal progenitor cells before and after culture under osteogenic conditions in perfusion bioreactors for 5 weeks.
Experiment type
transcription profiling by array 
Contacts
Giuseppe Maria de Peppo <gmdepeppo@nyscf.org>, Dana Alsalman, Darja Marolt, David Kahler, Gisueppe M de Peppo, Gordana Vunjak-Novakovic, Ivan M Campos, Linshan Shang
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-46315.idf.txt
Sample and data relationshipE-GEOD-46315.sdrf.txt
Processed data (1)E-GEOD-46315.processed.1.zip
Array designA-GEOD-10558.adf.txt
Links