E-GEOD-46314 - Development of gene expression signatures to characterize evolution of hASC cells isolated and maintained xenogeneic-free

Status
Released on 24 April 2013, last updated on 3 June 2014
Organism
Homo sapiens
Samples (4)
Array (1)
Protocols (6)
Description
Human adipose stem cells (hASCs) play a crucial role in the fields of regenerative medicine and tissue engineering for different reasons: the abundance of adipose tissue, their easy harvesting, the ability to multipotent differentiation and the fact that they do not trigger allogeneic blood response or secrete cytokines that act as immunosuppressants. The vast majority of protocols use animal origin reagents, with the underlying risk of transmitting infections by non-human pathogens. We have designed a protocol to isolate and maintain the properties of hASCs avoiding xenogeneic reagents. These changes not only preserve hASCs morphology, but also increase cell proliferation and maintain their stem cell marker profile. On the other hand, human serum albumin (HSA), Tryple® and human Serum (HS), do not affect hASCs multipotent differentiation ability. The amendments introduced do not trigger modifications in the transcriptional profile of hASCs, alterations in key biochemical pathways or malignization. Thus, we have proven that it is possible to isolate and maintain hASCs avoiding animal reagents and, at the same time, preserving crucial culture parameters during long term culture. Thereby we have revealed a novel and effective tool for the improvement of clinical, cell-based therapies. Gene expression in human adipose stem cells isolated and maintained with human reagents were measured immediately after their isolation, and at passages 1, 3 and 5. Pools from the same 8 healthy donors were used to measure the 4 points of growth.
Experiment type
transcription profiling by array 
Contacts
Andrés Sanz-García <sanz.andres@gmail.com>, Escobedo-Lucea Carmen, Sanz-Garcia Andres
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-46314.idf.txt
Sample and data relationshipE-GEOD-46314.sdrf.txt
Raw data (1)E-GEOD-46314.raw.1.zip
Processed data (1)E-GEOD-46314.processed.1.zip
Array designA-AGIL-28.adf.txt
Links