5 protocols
AccessionNameType
P-GSE46311-1
normalization data transformation protocol
The scanned images were processed using GCOS 1.4 (Affymetrix) according to the manufacturer. The resulting data sets (CEL files) were imported into ArrayAssist Expression Software (v5.2.0;Iobion Informatics LLC) and normalized using the PLIER algorithm in Array Assist to calculate relative signal values for each probe set. ID_REF = VALUE = Log2 PLIER signals
P-GSE46311-5
array scanning protocol
Signal intensities were detected by Affymetrix GeneChip Scanner 3000
P-GSE46311-4
hybridization protocol
The cRNA was hybridized to HG U133 plus 2.0 Arrays (Affymetrix) followed by washing and staining on the GeneChips Fluidics Station 450 (Affymetrix)
P-GSE46311-3
labelling protocol
Double-stranded cDNA and biotin-labeled cRNA probes were made from 5 μg of total RNA by use of GeneChip® Expression 3' Amplification One-Cycle Target Labeling kit (Affymetrix).
P-GSE46311-2
nucleic acid extraction protocol
Trizol extraction followed by Rneasy clean up kit (qiagen) was performed according to the manufacturer´s instruction