E-GEOD-46286 - Global Gene Expression Analysis of Term Amniotic Fluid Cell-Free Fetal RNA

Status
Released on 23 April 2013, last updated on 3 June 2014
Organism
Homo sapiens
Samples (16)
Array (1)
Protocols (5)
Description
The objective of this study was to identify the tissue expression patterns and biological pathways enriched in term amniotic fluid cell-free fetal RNA by comparing functional genomic analyses of term and second-trimester amniotic fluid supernatants. There were 2,871 significantly differentially regulated genes. In term amniotic fluid, tissue expression analysis showed enrichment of salivary gland, tracheal, and renal transcripts as compared with brain and embryonic neural cells in the second trimester. Functional analysis of genes upregulated at term revealed pathways that were highly specific for postnatal adaptation such as immune function, digestion, respiration, carbohydrate metabolism, and adipogenesis. Inflammation and prostaglandin synthesis, two key processes involved in normal labor, were also activated in term amniotic fluid. This was a prospective whole genome microarray study comparing eight amniotic fluid samples collected from eight women at term who underwent prelabor cesarean delivery and eight second-trimester amniotic fluid samples from routine amniocenteses. A functional annotation tool was used to compare tissue expression patterns in term and second-trimester samples. Pathways analysis software identified physiologic systems, molecular and cellular functions, and upstream regulators that were significantly overrepresented in term amniotic fluid.
Experiment type
transcription profiling by array 
Contacts
Andrea G Edlow, Diana W Bianchi, Heather C Wick, Janet M Cowan, Lisa Hui
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-46286.idf.txt
Sample and data relationshipE-GEOD-46286.sdrf.txt
Raw data (1)E-GEOD-46286.raw.1.zip
Processed data (1)E-GEOD-46286.processed.1.zip
Array designA-AFFY-44.adf.txt
Links