E-GEOD-46136 - Chromatin-to-nucleoprotamine transition is controlled by the histone H2B variant TH2B [Illumina]

Status
Released on 17 October 2013, last updated on 3 June 2014
Organism
Mus musculus
Samples (44)
Array (1)
Protocols (48)
Description
The conversion of male germ cell chromatin to a nucleoprotamine structure is fundamental to the life cycle yet the underlying molecular details remain obscure. Here we show that an essential step is the genome-wide incorporation of TH2B, a histone H2B variant of hitherto unknown function. Using mouse models in which TH2B is depleted or C-terminally modified we show that TH2B directs the final transformation of dissociating nucleosomes into protamine-packed structures. Depletion of TH2B induces compensatory mechanisms that permit histone removal by up-regulating H2B and programming nucleosome instability through targeted histone modifications, including lysine crotonylation and arginine methylation. Furthermore, after fertilization, TH2B re-assembles onto the male genome during protamine-to-histone exchange. Thus, TH2B is a unique histone variant, which plays a key role in the histone-to-protamine packing of the male genome and guides genome-wide chromatin transitions that both precede and follow transmission of the male genome to the egg. Total RNA were obtained from testes from meiotic (spermatocytes) and post-meiotic (round spermatids) male germ cells from adult wt and Th2b+/tag mice (experiment 1: 6 biological replicates for each condition) and adult wt and th2bkd mice (experiment 2: 5 biological replicates for each condition). In each experiment, 5 or 6 replicates of each genotype and each cell type were used. Th2b+/tag mice expressed TH2B C-terminally fused to three consecutive affinity tags: His, Flag and Ha. Th2bkd mice did not express any TH2B.
Experiment type
transcription profiling by array 
Contacts
Sophie Rousseaux <sophie.rousseaux@ujf-grenoble.fr>, Alexandra Debernardi, Emilie Montellier, Fayçal Boussouar, Matthieu Gérard, Philippe Guardiola, Saadi Khochbin
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-46136.idf.txt
Sample and data relationshipE-GEOD-46136.sdrf.txt
Processed data (1)E-GEOD-46136.processed.1.zip
Array designA-MEXP-1175.adf.txt
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