E-GEOD-46135 - Epigenome analysis of DNA modification 5-hydroxymethylcytosine in normal, abnormal, and globozoospermia sperm genomes

Status
Released on 18 April 2013, last updated on 12 June 2013
Organism
Homo sapiens
Samples (3)
Protocols (2)
Description
Genome wide of 5-hydroxymethylcytosine profiling of normal and abnormal, and globozoospermia sperm genomes.Two semen samples were collected from a healthy man and a globozoospermia patient who had consulted a physician at ShengJing Hospital of China Medical University. Other semen samples obtained from two volunteers who were good health generally. 5-hmC enriched genomic DNA libraries were generated following the Illumina protocol for “Preparing Samples for CHIP sequencing of DNA”. 100bp single end sequencing on Illumina Hiseq2000 to get 5-hmC-enriched DNA fragment sequence was performed. Examination of 5hmC levels in normal, abnormal, and globozoospermia sperm genomes
Experiment type
methylation profiling by high throughput sequencing 
Contacts
sun baofa <baofa1983@126.com>, Xiu-Xia Wang, You-Qi Liao, Yun-Gui Yang
MINSEQE
Exp. designProtocolsFactorsProcessedSeq. reads
Files
Investigation descriptionE-GEOD-46135.idf.txt
Sample and data relationshipE-GEOD-46135.sdrf.txt
Processed data (1)E-GEOD-46135.processed.1.zip
Links