7 protocols
AccessionNameType
P-GSE46112-1
normalization data transformation protocol
Primary analysis was carried out by Functional Genomics Core Facility in UNC-CH using Affymetrix Expression Console software. probe group file: MoGene-1_0-st-v1.r4.pgf meta-probeset file: MoGene-1_0-st-v1.r4.mps ID_REF = VALUE = RMA normalized signal intensity
P-GSE46112-7
array scanning protocol
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G by Functional Genomics Core Facility in UNC-CH using standard protocol.
P-GSE46112-6
hybridization protocol
Hybridization to Affymetrix MoGene-1_0-st-v1 array was carried out by Functional Genomics Core Facility in UNC-CH using standard protocol.
P-GSE46112-5
labelling protocol
Biotin labeling was carried out by Functional Genomics Core Facility in UNC-CH using GeneChip WT Sense Target Labeling and Control Reagents and standard protocol.
P-GSE46112-3
growth protocol
E14 mESCs are cultured on the 0.1% gelatin coated plates in the DMEM medium (Sigma) supplemented with 100U/ml penicillin/streptomycin, 15% fetal bovine serum (Sigma), 1x nonessential amino acid, 1x sodium pyruvate, 1x GlutaMax, 1x beta-mercaptoethanol (Invitrogen) and 1000 units/ml leukemia inhibitory factor (ESGRO, EMD Millipore).
P-GSE46112-2
sample treatment protocol
Around 1x10^5 ES cells/well in 24-well plates are infected with lentiviral vectors at 10 MOI. 48 hours after transduction, puromycin (2ug/ml) is added to the medium for selecting transduced cells.
P-GSE46112-4
nucleic acid extraction protocol
Total RNA was extracted from the control and Kdm2b knockdown mESCs using RNeasy mini kit (QIAGEN) following manufacture's instructions.