7 protocols
AccessionNameType
P-GSE45955-1
normalization data transformation protocol
CEL files were generated from images (DAT files) using Command Console (Affymetrix, Santa Clara, CA), log2 RMA expression estimates were calculated using Partek Genomics Suite ID_REF = VALUE = log2 RMA expression estimate
P-GSE45955-7
array scanning protocol
GeneChips were scanned in a GeneAtlas Imaging Station (Affymetrix, Santa Clara, CA)
P-GSE45955-6
hybridization protocol
8 µg of cDNA was hybridized per microarray for 16 hours of hybridization at 45ºC, washing and staining of microarrays was performed using a GeneAtlas Fluidics Station (Affymetrix, Santa Clara, CA)
P-GSE45955-5
labelling protocol
RNA was amplified for 23 cycles using the TransPlex Complete Whole Transcriptome Amplification Kit (Sigma; reference WTA2) and subsequently labeled using GeneChip Mapping 250K Nsp Assay Kit (Affymetrix; catalog # 900766), as described in Gonzalez Roca et al. 2012.
P-GSE45955-3
growth protocol
freshly isolated progenitor cells from lineage depleted bone marrow of ID2-GFP reporter were analysed. Cells were not cultivated prior RNA isolation.
P-GSE45955-4
nucleic acid extraction protocol
RNA was extracted using Agencourt RNAClean XP magnetic beads (Beckman Coulter) as described (Gonzalez Roca et al. 2010)
P-GSE45955-2
sample treatment protocol
cells were flow cytometrically isolated from lineage depleted bone marrow derived from ID2-GFP reporter mice and immediately lysed for RNA purification.