E-GEOD-45955 - Differential requirement for Nfil3 during NK cell development

Status
Released on 8 October 2013, last updated on 3 June 2014
Organism
Mus musculus
Samples (8)
Array (1)
Protocols (7)
Description
Nfil3 has multiple functions during conventional NK cell and thymic NK cell development. Nfil3 controls NK cell development from the earliest committed NK cell progenitor stage. Nfil3-/- prepro NK cells retained T-cell potential due to the failure to repress T cell specific genes. At later stages of NK cell development, Nfil3 regulates the formation of mature NK cells by promoting directly or indirectly the expression of transcription factor Eomes. We also demonstrate that Nfil3 is important for thymic NK cell development whereas Trail+ immature NK cells generated in the bone marrow of neonates and in the adult liver develop independent of Nfil3 function. We used microarrays to analyse the changes in gene expression due to the presence or absence of Nfil3, both in prepro NK cells and ALP cells (all lymphoid progenitors) Samples were obtained on two experimentation dates. Replicate 1 was processed in April 2012, replicate 2 was processed in May 2012.
Experiment type
transcription profiling by array 
Contacts
Victoria Core <victoria@dnaarrays.org>, Cyril Seillet, Sebastian Carotta
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-45955.idf.txt
Sample and data relationshipE-GEOD-45955.sdrf.txt
Raw data (1)E-GEOD-45955.raw.1.zip
Processed data (1)E-GEOD-45955.processed.1.zip
Array designA-GEOD-11180.adf.txt
Links