E-GEOD-45808 - Phrenic neuron determinant gain-of-function screen in M. musculus ES cell-derived motor neurons

Status
Released on 1 October 2013, last updated on 7 October 2013
Organism
Mus musculus
Samples (72)
Array (1)
Protocols (7)
Description
Expression response after induction of putative phrenic neuronal determinants in ES cell-derived motor neurons was compared to a pre-determined list of genes over-expressed in FACS-sorted primary. Transcription factor Pou3f1 was identified as a major determinant of phrenic identity. Expression in induced cell lines were compared to YFP controls, and over-representation of phrenic genes was computed for the list of differentially expressed genes in each indiced cell lines.
Experiment type
transcription profiling by array 
Contacts
Eric Blanc <eric.blanc@kcl.ac.uk>, Carolina Barcellos-Machado, Ivo Lieberam
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-45808.idf.txt
Sample and data relationshipE-GEOD-45808.sdrf.txt
Raw data (2)E-GEOD-45808.raw.1.zip, E-GEOD-45808.raw.2.zip
Processed data (1)E-GEOD-45808.processed.1.zip
Array designA-AFFY-130.adf.txt
Links