7 protocols
AccessionType
image_aquisition
Standard Illumina scan protocol
feature_extraction
The data were normalized by average signal on chip with Illumina BeadStudio (v.3.1.3.0).
bioassay_data_transformation
ID_REF = VALUE = BeadStudio computed normalized signal intensity. Detection Pval =
hybridization
Standard Illumina hybridization protocol
labeling
cRNA was labeled with biotin using Illumina TotalPrep RNA Amplification kit (Ambion)
specified_biomaterial_action
The mice were treated with vehicle or S961 (10nMol/week) using Alzet osmotic pump for a week.
nucleic_acid_extraction
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit.