E-GEOD-45475 - The formation of the intestine at the juvenile stage is raised by the Hox10 gene through the migration of endodermal strand cells in ascidian

Status
Released on 1 October 2013, last updated on 3 June 2014
Organism
Ciona intestinalis
Samples (4)
Array (1)
Protocols (7)
Description
Hox cluster genes play crucial roles in the establishment of the body plan along the antero-posterior axis during animal development. Hox genes are expressed in the chordate endodermal tissues, and unraveling functions there is necessary for elucidating the mechanisms of endoderm specification. In the invertebrate chordate Ciona intestinalis, the endodermal tissues are in the premature state at the larval stage, and they form the differentiated digestive tract during metamorphosis. In this study, we showed that a Hox gene Ci-Hox10 is required for the formation of the intestine during metamorphosis. To know the downstream genes that are controlled by Hox10, microarray analysis of Hox10 knock-down embryos was performed. Gene expressions in the tail region of Hox10 knockdown embryos that were injected with Hox10 morpholino (MO) and control embryos of Ciona intestinalis were examined by comparative analysis with two color detection. Dye-swap analysis was carried out.
Experiment type
transcription profiling by array 
Contacts
Mayuko Hamada <hamadam@oist.jp>, Hidetoshi Saiga, Narudo Kawai, Nori Satoh, Tetsuro Ikuta, Yasunori Sasakura, Yosuke Ogura
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-45475.idf.txt
Sample and data relationshipE-GEOD-45475.sdrf.txt
Raw data (1)E-GEOD-45475.raw.1.zip
Processed data (1)E-GEOD-45475.processed.1.zip
Array designA-GEOD-5576.adf.txt
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