E-GEOD-45466 - Dynamic genome-wide transcriptional response of human monocyte-derived dendritic cells to IL-6 and Il-10

Status
Released on 26 March 2013, last updated on 3 June 2014
Organism
Homo sapiens
Samples (48)
Array (1)
Protocols (8)
Description
Single cell timecourse experiments demonstrated that though IL-6 and IL-10 activate STAT3, they generate different temporal patterns of activation, with IL-6 generating a transient activation of STAT3, and IL-10 generating a sustained STAT3 response. We hypothesized that these different STAT3 activation dynamics would dictate the differing cellular responses to the two cytokines. Human monocyte-derived denditic cells from four different donors were treated with IL-6 or IL-10 for either 0min, 45min, 2hr, 4hr, 8hr, or 12hr. Total RNA was isolated from each sample and used for gene expression analysis.
Experiment type
transcription profiling by array 
Contacts
David Braun <david.braun@mssm.edu>, David A Braun, Miguel Fribourg, Stuart C Sealfon
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-45466.idf.txt
Sample and data relationshipE-GEOD-45466.sdrf.txt
Raw data (1)E-GEOD-45466.raw.1.zip
Processed data (1)E-GEOD-45466.processed.1.zip
Array designA-GEOD-10558.adf.txt
Links