E-GEOD-45437 - Expression data from paediatric ependymoma short-term cell cultures

Status
Released on 2 December 2013, last updated on 3 June 2014
Organism
Homo sapiens
Samples (12)
Array (1)
Protocols (7)
Description
Promoter hypermethylation and transcriptional silencing is a common epigenetic mechanism of tumour suppressor inactivation in cancer, including malignant brain tumours. To identify targets of epigenetic silencing mediated by CpG island methylation in paediatric ependymoma, we used a pharmacological unmasking approach through treatment with the demethylating agent 5-Aza-2’-deoxycytidine followed by global expression microarray analysis. Three short-term ependymoma cell cultures were used for whole genome expression analysis following treatment with the demethylating agent 5-Aza-dC (5 µmol/L) or mock-treated with DMSO (≤0.1% v/v) for 96-hrs.
Experiment type
transcription profiling by array 
Contact
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-45437.idf.txt
Sample and data relationshipE-GEOD-45437.sdrf.txt
Raw data (1)E-GEOD-45437.raw.1.zip
Processed data (1)E-GEOD-45437.processed.1.zip
Array designA-AFFY-44.adf.txt
Links