7 protocols
AccessionNameType
P-GSE45430-1
bioassay_data_transformation
ID_REF = VALUE = normalized intensity
P-GSE45430-7
feature_extraction
The data were analyzed using RMA in the bioconducter and normalized using the Cross Correlation method. The trimmed mean target intensity of each array was set to 8.
P-GSE45430-6
image_aquisition
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
P-GSE45430-5
hybridization
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
P-GSE45430-4
labeling
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
P-GSE45430-2
grow
All mice were kept in a sterile barrier facility approved by the Beth Israel Deaconess Medical Center Institutional Animal Care and Use Committee.
P-GSE45430-3
nucleic_acid_extraction
Cells using the markers (CD45.1−CD45.2+Lin*–Sca-1-Mac1lo/+c-Kit+) were collected after sorting and placed on ice in the Trizol solution (GibcoBRL). Trizol extraction of total RNA was performed according to the manufacturer's instructions.