E-GEOD-45430 - Sox4 is a key oncogenic target in C/EBPα mutant Acute Myeloid Leukemia

Status
Released on 23 March 2013, last updated on 3 June 2014
Organism
Mus musculus
Samples (9)
Array (1)
Protocols (7)
Description
Mutation or epigenetic silencing of the transcription factor C/EBPα is observed in ~10% of patients with acute myeloid leukemia (AML). In both cases, a common global gene expression profile is observed, but down-stream targets relevant for leukemogenesis are not known. Here we identify Sox4 as a direct target of C/EBPα whereby its expression is inversely correlated with C/EBPα activity. Downregulation of Sox4 abrogated increased self-renewal of leukemic cells and restored their differentiation. Gene expression profiles of leukemia initiating cells (LICs) from both Sox4 overexpression and murine mutant C/EBPα AML models clustered together, but differed from other types of AML. Our data demonstrate that Sox4 overexpression resulting from C/EBPα inactivation contributes to the development of leukemias with a distinct LIC phenotype. K/L (bi-allelic Cebpa mutations) leukemic mice and Sox4 overexprssing leukemic mice were used for RNA extraction and hybridization on Affymetrix microarrays. We compared these microarray samples with the C57/BL6 wild type mice.
Experiment type
transcription profiling by array 
Contacts
Borja Saez, Constanze Bonifer, Daniel G Tenen, David T Scadden, Giovanni Amabile, Henry Yang, Hong Zhang, Junyan Zhang, Maarten Hoogenkamp, Meritxell Alberich-Jordà, Min Ye, Peter J Valk, Philipp B Staber, Rob Welner, Ruud Delwel, Véronique Lefebvre
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-45430.idf.txt
Sample and data relationshipE-GEOD-45430.sdrf.txt
Raw data (1)E-GEOD-45430.raw.1.zip
Processed data (1)E-GEOD-45430.processed.1.zip
Array designA-AFFY-45.adf.txt
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