E-GEOD-45393 - Facilitates chromatin transcription complex is a marker and target of aggressive cancers with lower survival rates

Status
Released on 22 March 2013, last updated on 4 April 2013
Organism
Homo sapiens
Samples (12)
Protocols (4)
Description
The Facilitates Chromatin Transcription (FACT) is involved in chromatin remodeling during transcription, replication and DNA repair and is considered to be an ubiquitously expressed complex that has no known associations with any disease. However, we discovered that FACT is expressed in very limited number of cells of adult mammalian organism, mostly presented by stem and undifferentiated cells. Here, we show that FACT is present in poorly differentiated aggressive cancers with an overall low survival rate. FACT acts as an "accelerator" of tumor transformation. It cannot drive transformation like an oncogene, but it increases the efficiency of oncogene-induced transformation. FACT expressing cancer cells cannot grow in the absence of FACT possibly due to the FACT involvement in selective chromatin remodeling of genes that stimulate proliferation, inhibit cell death and differentiation and that are induced in response to cell stress. We propose that FACT is a marker of an aggressive cancer phenotype and Patients with FACT-positive tumors are noted for an overall poorer survival rate. FACT expression in primary tumors may be used as a predictor of metastatic disease. FACT may also be used as a target of anti-cancer therapy because tumor cells expressing FACT are dependent on FACT function, while normal cells either do not express FACT or are not sensitive to FACT inhibition. Examination of AR binding sites of 3 replicates of untreated cells and 3 replicates of curaxin-treated cells, each with 3 replicate controls
Experiment type
methylation profiling by high throughput sequencing 
Contacts
Robert William Leach <rwleach@ccr.buffalo.edu>, Alfiya Safina, Angela R Omilian, Anja Ruusulehto, Carl Morrison, Henry Garcia, Jeffrey C Miecznikowski, Jianmin Wang, Katerina Gurova, Mairead Commane, Michael Buck, Olga Guryanova, Olympia Papantonopoulou, Robert W Leach, Sami Kilpinen, Seamus Degan, Song Liu, Yan Li
MINSEQE
Exp. designProtocolsFactorsProcessedSeq. reads
Files
Investigation descriptionE-GEOD-45393.idf.txt
Sample and data relationshipE-GEOD-45393.sdrf.txt
Processed data (1)E-GEOD-45393.processed.1.zip
Links