E-GEOD-45378 - HDAC mediated suppression of histone turnover promotes epigenetic stability of heterochromatin

Status
Released on 21 April 2013, last updated on 2 June 2014
Organism
Schizosaccharomyces pombe
Samples (26)
Arrays (2)
Protocols (8)
Description
Analysis of histone H3 turnover in wild-type and mutant fission yeast cells by using MNase-ChIP-chip Exponentially growing cultures of fission yeast cells expressing an ectopic copy of FLAG tagged histone H3 under the control of inv1 promoter were synchronized and expression of H3-FLAG was induced by changing the carbon source of the medium to Sucrose. Cells were crosslinked with 1% Formaldehyde and chromatin was fragmented into mononucleosomes by using micrococcal nuclease (MNase). Chromatin immunoprecipitated DNA recovered with anti-FLAG antibody from wild-type and mutant fission yeast cells and whole cell extracts DNA were amplified by random priming and respectively labeled with Cy5 and Cy3. Labeled DNA samples were mixed and hybridized onto 44k pombe Agilent oligo arrays. Data were processed using Agilent scanner and Feature Extraction software.
Experiment type
ChIP-seq 
Contacts
Shiv Grewal <grewals@mail.nih.gov>, Ozan Aygün, Sameet Mehta, Shiv I Grewal
MINSEQE
Exp. designProtocolsVariablesProcessedSeq. reads
MIAME
PlatformsProtocolsVariablesProcessedRaw
Files
Investigation descriptionE-GEOD-45378.idf.txt
Sample and data relationshipE-GEOD-45378.sdrf.txt
Raw data (1)E-GEOD-45378.raw.1.zip
Processed data (1)E-GEOD-45378.processed.1.zip
Array designsA-GEOD-11244.adf.txt, A-GEOD-16854.adf.txt
Links