E-GEOD-4536 - Transcription profiling of human tumor stem cells (TSC) and their matched glioma cells lines reveals TSCs more closely mirror the phenotype and genotype of primary human tumors than do cancer cell lines
Submitted on 24 March 2006, released on 13 June 2008, last updated on 27 March 2012
The concept of tumor stem cells (TSCs) provides a new paradigm for understanding tumor biology, although it remains unclear whether TSCs will prove to be a more robust model than traditional cancer cell lines. We demonstrate marked phenotypic and genotypic differences between primary human tumor-derived TSCs and their matched glioma cell lines. TSCs derived directly from primary glioblastomas harbor extensive similarities to normal NSC and recapitulate the genotype, gene expression patterns and in vivo biology of human glioblastomas. By contrast, the matched, traditionally grown tumor cell lines do not secondary to in vitro genomic alterations. These findings suggest that TSCs may be a more reliable model than many commonly utilized cancer cell lines for understanding the biology of primary human tumors. Analysis of gene expression data is described in Lee et al., Cancer Cell, 2006. Experiment Overall Design: To further understand the differences between NBE- and serum-cultured GBM cells, we generated gene expression profiles of NBE-cells, serum-cells, their derived xenograft tumors, and the original GBMs taken directly from the patients. NBE- and serum-cultured GBM cells from different passages were also included in the analyses in order to evaluate the potential effects of in vitro passage number on gene expression.
transcription profiling by array, unknown experiment type
Tumor stem cells derived from glioblastomas cultured in bFGF and EGF more closely mirror the phenotype and genotype of primary tumors than do serum-cultured cell lines. Jeongwu Lee, Svetlana Kotliarova, Yuri Kotliarov, Aiguo Li, Qin Su, Nicholas M Donin, Sandra Pastorino, Benjamin W Purow, Neil Christopher, Wei Zhang, John K Park, Howard A Fine. Cancer Cell 9(5):391-403 (2006)