E-GEOD-45336 - Genome-wide methylation and expression analysis of two breast cancer cell lines [Methyl-MAPS]

Status
Released on 15 June 2013, last updated on 4 July 2013
Organism
Homo sapiens
Samples (2)
Protocols (4)
Description
To improve our understanding of the relationships between methylation and expression we profiled mRNA expression and single-base resolution methylation levels for two breast cancer cell lines, MCF7 and T47D. Expression was profiled using RNA-seq. Methylation was assayed using Methyl-MAPS, which uses methylation-sensitive and -dependent restriction enzyme digests followed by high-throughput sequencing to identify methylation levels at individual CpGs (Edwards et al. 2010, Genome Research). DNA Methylation was assayed for two breast cancer cell lines using Methyl-MAPS.
Experiment type
methylation profiling by high throughput sequencing 
Contacts
Nathan VanderKraats <ndvk1980@yahoo.com>, Jeffrey F Hiken, John R Edwards, Nathan D VanderKraats
Citation
MINSEQE
Exp. designProtocolsFactorsProcessedSeq. reads
Files
Investigation descriptionE-GEOD-45336.idf.txt
Sample and data relationshipE-GEOD-45336.sdrf.txt
Processed data (1)E-GEOD-45336.processed.1.zip
Links