E-GEOD-45325 - RNAseq analysis of the serotype M1 group A Streptococcus strain MGAS2221

Status
Released on 20 March 2013, last updated on 4 April 2013
Organism
Streptococcus pyogenes
Samples (4)
Protocols (3)
Description
We recovered RNA from exponential and stationary phase cell cultures of MGAS2221 and utilized two distinct methods to remove rRNA from our samples, the RiboZero kit from Epicenter, and the terminator- 5--Phosphate-Dependent Exonuclease (TEX). This gave us 4 distinct RNAs with which we performed RNAseq analysis. Both rRNA removal methods worked well, with only a small percentage of the final total read counts being derived from rRNA sequences. A single group A Streptococcus strain was investigated.
Experiment type
RNA-seq of coding RNA 
Contacts
MINSEQE
Exp. designProtocolsFactorsProcessedSeq. reads
Files
Investigation descriptionE-GEOD-45325.idf.txt
Sample and data relationshipE-GEOD-45325.sdrf.txt
Links