E-GEOD-4532 - Transcription profiling of human samples differing in hemoglobin transcripts to investigate cross-hybridization comparison between cRNA target and cDNA target

Status
Submitted on 24 March 2006, released on 13 June 2008, last updated on 10 June 2011
Organism
Homo sapiens
Samples (12)
Array (1)
Protocols (4)
Description
We used two RNA samples that differed only by a set of three hemoglobin transcripts to compare microarray cross-hybridization between two target preparation protocols. We found widespread cross-hybridization using standard cRNA target but substantially less cross-hybridization using cDNA target. Experiment Overall Design: 2 protocols x 2 RNA samples x 3 replicates = 12 arrays.
Experiment types
transcription profiling by array, unknown experiment type
Contact
Aron C. Eklund
MIAME
PlatformsProtocolsFactorsProcessedRaw
Files
Investigation descriptionE-GEOD-4532.idf.txt
Sample and data relationshipE-GEOD-4532.sdrf.txt
Raw data (1)E-GEOD-4532.raw.1.zip
Processed data (1)E-GEOD-4532.processed.1.zip
Array designA-AFFY-37.adf.txt
R ExpressionSetE-GEOD-4532.eSet.r
Links