E-GEOD-45276 - Expression data from Control, AKT and RAS transduced cells
Released on 19 March 2013, last updated on 2 June 2014
Mutations in both RAS and the PTEN/PIK3CA/AKT signaling module are found in the same human tumors. PIK3CA and AKT are downstream effectors of RAS, and the selective advantage conferred by mutation of two genes in the same pathway is unclear. Based on a comparative molecular analysis, we show that activated PIK3CA/AKT is a weaker inducer of senescence than is activated RAS. More-over, concurrent activation of RAS and PIK3CA/AKT impairs RAS-induced senescence. We used microarrays to detail the global programme of gene expression after transduction of AKT and RAS IMR90 cells were transfected with Control, AKT and RAS retrovirus containing medium in 4 replicates. Fibroblasts were drug selected and kept in drug for duration of experiments.
transcription profiling by array
Alyssa L Kennedy, Brendan Doyle, David M Nelson, Greg H Enders, Indrani Manoharan, Jeff S Pawlikowski, Jennifer P Morton, Karin A Oien, Nigel B Jamieson, Owen J Sansom, Peter D Adams, Rugang Zhang, Tony McBryan
Activation of the PIK3CA/AKT pathway suppresses senescence induced by an activated RAS oncogene to promote tumorigenesis. Kennedy AL, Morton JP, Manoharan I, Nelson DM, Jamieson NB, Pawlikowski JS, McBryan T, Doyle B, McKay C, Oien KA, Enders GH, Zhang R, Sansom OJ, Adams PD. , Europe PMC 21474066